Key points: DNA cloning is a molecular biology technique that makes many identical copies of a piece of DNA, such as a gene. In a typical cloning experiment, a target gene is inserted into a circular piece …

In DNA cloning, researchers make many copies of a piece of DNA, such as a gene. In many cases, cloning involves inserting the gene into a piece of circular DNA called a plasmid, which can be …

In a typical cloning experiment, researchers first insert a piece of DNA, such as a gene, into a circular piece of DNA called a plasmid. This step uses restriction enzymes and DNA ligase and is called a …

Although genomes are now typically sequenced using other methods that are faster and less expensive, Sanger sequencing is still in wide use for the sequencing of individual pieces of DNA, such as …

DNA cloning involves creating identical copies of a specific gene using restriction enzymes to cut the desired gene, pasting it into a plasmid, and inserting it into bacteria like E. coli. The bacteria replicate …

Khan Academy ... Khan Academy

Introduction Suppose you have just done a PCR reaction, making many copies of a target DNA region. Or perhaps you’ve done some DNA cloning, trying to "paste" a gene into a circular DNA plasmid. …

During DNA replication, the two strands of the DNA double helix are unwound and separated by enzymes. Each strand then serves as a template, or guide, for synthesizing a new, complementary …

DNA helps make us who we are, but how exactly does it work? In this unit, we'll examine the nitty gritty of replication, transcription, and translation, and learn how seemingly small mutations can have a big …

Restriction digestion. Sticky ends and blunt ends. Ligation reactions.